It's the final day of trading for the Mansfield branch of the Nottinghamshire based company that went into administration in August.
Earlier this month, Wilko’s administrators PwC announced the closure of all stores with Mansfield being one of the 111 scheduled to close week commencing Monday, September 25.
The Sutton branch closed on Tuesday.
The company was founded in 1930 in Leicester and went on to open more than 400 stores across the UK.